Canine herpesvirus (CHV-1) causes disease associated with high mortality in infected\npuppies, which represents large financial losses for dog breeders. Since CHV-1\nat the time of the study he had not been reported in Mexico, the main objective of\nthis study was to determine the prevalence of antibodies against CHV-1 in canine\nkennels in the metropolitan area of Mexico City. A commercial enzyme-linked immunosorbent\nassay (ELISA) was used, and the results were compared to those of a\nviral neutralization test. The ELISA kit uses the complete viral particle as the antigen.\nThe plaque reduction neutralization test was combined with the immunoperoxidase\ntechnique because of the low cytopathic effect of CHV-1. Neutralizing antibodies\nwere also detected in 20 randomly selected samples. The prevalence of CHV-1 with\nELISA was 87%. The concordance between ELISA and serum neutralization (SN)\nwas 0.1129, the sensitivity of the ELISA against SN was 1.0 (100%), the positive predictive\nvalue was 0.39 (39%), and the negative predictive value was 1 (100%). These\nresults show that ELISA is useful for monitoring the dog population for CHV-1; a\npositive test result requires confirmation with an SN test, and a negative ELISA result\nindicates a high probability of being SN-negative. The only variables that were statistically\nassociated with CHV-1 prevalence were breed and kennel. A statistically significant\nrelationship between the degree of ELISA and SN titer was obtained, with a\nconfidence level of 95%. None of the clinical presentation factors was statistically\nsignificant. These results suggest that most of the canine population studied in Mexico\nis in a herpesvirus latency state.
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